HDcryoNet provides state-of-the-art cryo-EM infrastructure at BioQuant and the Biochemistry Center (BZH). It fosters collaborative research and operates as a user-driven network that enables independent access to equipment with shared responsibilities among its members. The network operates four high-end cryo-EM instruments to support cutting-edge scientific exploration, two of them located at BioQuant:

• Glacios – cryo-TEM (200kV): Cutting-edge 200kV cryo-transmission electron microscope that allows sample screening and data collection in single-particle analysis of purified complexes. It features an Autoloader, a field emission gun, a Falcon3-EC direct detector, and a Volta phase plate. Remote data collection is enabled via EPU or SerialEM. Located near the Talos L120C microscope, it supports single-particle projects. Acquired through DFG funding and in operation since 2020.
• Talos L120C – (cryo)-TEM 120 kV: State-of-the-art 120kV transmission electron microscope that can be used for cryogenic temperature sample screening. It features a LaB₆ electron source, a Ceta-M camera (4k × 4k CMOS sensor), and supports multiple side-entry holders, including the Elsa Cryo-Transfer Holder. Funded by the DFG and in operation since 2019.

For sample praparation, we also have two automatic plunge freezers (Vitrobot Mark IV and Gatan Cryoplunge 3) as well as a glow discharger (Gatan Solarus 950).